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Professor James N Miller
Emeritus Professor of Analytical Chemistry,
MA, PhD, Cantab, CChem, FRSC
Telephone: ++44 (0)1509 222570
FAX: ++44 (0)1509 223925
Email: J.N.Miller@lboro.ac.uk
Analytical Chemistry Interests
Fluorescence Spectroscopy:
Fluorimetric analysis methods are distinguished by their exceptional sensitivity,
unusual selectivity, and convenient combination with other techniques
such as chromatography, immunoassays, etc. We are interested in
many aspects of fluorescence analysis, including the synthesis of new
label and probe molecules; long wavelength/near-ir instrumentation and
techniques; structure-spectra correlations in organic fluorophores; fluorescence
standards (materials and methods); and the related techniques of phosphorescence
and luminescence analysis.
Biospecific Analysis Methods:
Biochemical analysis presents unique problems of specificity, which are
often best overcome by the use of biochemical molecules as reagents. We
have a long-standing interest in the development of both homogeneous and
heterogeneous immunoassays using fluorescence, chemiluminescence and enzyme
labels; the application of immobilised enzymes and other solid phase biochemical
reagents; and the characterisation of drug-protein and other ligand binding
interactions.
Flow Injection Analysis:
Flow injection analysis is an approach to automatic analysis which allows
unique flexibility of experimental layout, sample and reagent dispersion,
and detection system. We are using it to automate immunoassays, characterise
protein binding interactions rapidly, carry out on-line solvent extractions,
and to provide robust and portable sensors.
Environmental Chemistry Interests
Our principal interest is in the use of immunoassays in the ultra-sensitive
detection of environmental pollutants, especially pesticides in river-
and ground-waters, and in the detection of antibiotics in foodstuffs,
especially meat and milk. Techniques used involve heterogeneous (i.e.
solid-phase) immunoassays with long-wavelength FL or CL detection.
Selected Publications
Sarpara, G.H., Hu, S.J., Palmer, D.A., French, M.T., Evans, M. and Miller,
J.N., ''A new long wavelength fluorigenic substrate for alkaline phosphatase:
synthesis and characterisation'', Analytical Communications, 36,
1999, pp 19-20, ISSN 1359-7337 .
Miller, J.N., Evans, M., French, M.T. and Palmer, D.A., ''Injection Based
Heterogeneous Fluorescence Immunoassays'', Applied Fluorescence in
Chemistry, Biology and Medicine, W. Rettig (ed), Springer, (Berlin),
1999, 509-519, ISBN 3-540-64451-2 .
Briggs, M.S.J., Bruce, I., Miller, J.N., Moody, C.J., Simmonds, A.C. and
Swann, E., ''Synthesis of functionalised fluorescent dyes and their coupling
to amines and amino acids'', Journal of the Chemical Society, Perkin
Transactions I, 1, 1997, 1051-1058, ISSN 0300-922X .
Guo, J.C., Miller, J.N., Evans, Mark and Palmer, D.A., ''Dual analyte
flow injection fluorescence immunoassays using thiophilic gel reactors
and synchronous scanning detection'', The Analyst, 125, 2000,
pp 1707-1708, ISSN 0003-2654 .
Khokhar, M.Y., Miller, J.N. and Seare, N.J., ''Heterogeneous Fluorescence
Immunoassays using Flow Injection Analysis with Protein G Solid Phase
Reactors'', Analytical Chimica Acta, 1994, 290, 154-158.
Palmer, D.A., Evans, M., Miller, J.N. and French, M.T., ''Rapid Fluorescence
Flow Injection Immunoassay Using a Novel Perfusion Chromatographic Matrix'',
Analyst, 1994, 119, 943-947.
Palmer, D.A., Evans, M. and Miller, J.N., ''Thiophilic Gels: and Alternative
to Protein A and G for Use in Flow Injection Immunoassays'', Analytical
Proceedings, 1994, 31, 123-125.
Hindocha, R.K., Miller, J.N. and Seare, N.J., ''On-Line Liquid-Liquid
Extraction with Near-Infrared Fluorescence Detection'', Analytical
Proceedings, 1994, 31, 185-186.
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